6X Laemmli buffer

Laemmli is a sample buffer to use in western blot.  6X sample buffer is added to each protein sample and is boiled or heated for 5-10 minutes. Recipe to prepare 10 ml: – 1.2gr SDS (sodium dodecyl sulfate) – 6mg bromophenol blue – 4.7ml glycerol – 1.2ml Tris 0.5M pH6.8 – 2.1ml dwater warm it […]