1. Place bottles in a 56oC water bath. Make sure the bottles cannot tip over or otherwise become submerged in the water bath. The temperature is critical for complement degredation, so make sure!
2. Wait until the temperature stabilizes at 56oC (after adding the serum to the water bath)
3. Gently swirl the bottles every 10 minutes [...]
The Department of Biological sciences of the University of Maryland (UMBC) has a protocol section very useful for cell culture first time users.
They explain extensively all the components of the work area and the equipment mainly used, from how the hood works to the microscope or the incubators. Also, types of cells that grow in [...]
Sigma-Aldrich publishes in its learning center web site a very complete guide to cell culture techniques.
It goes from the basic techniques to sterile techniques, freeze and thaw cells, culturing of semi-adherent or suspension cell lines, test for bacteria or fungi in the cultures, detection of mycoplasma,…
In summary, an extensive guide for cell culture beginners. Here [...]