Protocol for Poly-D-Lysine Preparation and Plate Coating

1. Prepare Poly-D-Lysine
a. Add 100 ml of sterile tissue culture grade H2O to 5 mg poly-D-lysine (Sigma P6407). Final concentration of poly-D-lysine is 50 ug/ml.
b. Mix by pipetting several times.
c. Store at 2-8 ºC or –20 ºC.
2. Prepare Coated Plates
a. Add enough poly-D-lysine solution to cover culture plate surface.
b. Incubate at room temperature for 1-24 [...]

Posted Under: Cell culture
This post was written by admin on May 13, 2009 Comments (0)

10X TBS (Tris Buffered Saline)

To prepare 1 liter of 10X TBS:
30 gr  Tris
88 gr NaCl
2 gr KCl
mix everything in dwater until it’s disolved. Adjust pH at 7.5

Posted Under: Buffers & recipes
This post was written by admin on May 12, 2009 Comments (0)

Paraformaldehide 4%

To prepare 100ml of PFA 4% stock solution:
- Add 86ml ddwater in an enlermeyer
- Boil water in the microwave
- Weigh 4g of PFA
From now on in the fume hood
- Transfer PFA in the erlenmeyer
- Add a clean stirring bar
- Add a drop of NaOH 10M
- Stir it and keep it hot (heat at #1-2) till it goes [...]

Posted Under: Buffers & recipes
This post was written by admin on May 1, 2009 Comments (0)