You know you’ve worked too long in a lab when

Very funny! and very true! hehehe. I got it from a friend in facebook. There is this facebook group called You know you’ve worked too long in a lab when, Daniel Sutton from London is the admin, but I don’t really know who is/are the author/authors of these hilarious statements. LOL 1. You wonder what absolute […]

Saving time and money doing maxi/midi preps

Here is a trick. It is very simple, only a bit messy. But it will save you one centrifugation step and 30 minutes. You have to pellet your overnight culture as usual, ressuspend it, lysate it and stop the lysis. So far, as recommended in the protocol. The next step is normally a centrifugation to […]

George W. Bush contribution to science

Ex-president George W. Bush inesperately contributed to science when he was able to duck flying shoes thrown at him by an Iraqi reporter in December in Bagdad as reveals the study published this month in the magazine Current Biology by two neurologists of Washington University. Bush reflexes and the impassivity that al-Maliki, the Iraqi primer […]

Most commonly used lysis buffers

RIPA 150 mM NaCl 1 mM PMSF 1 mM EDTA 5 µg/ml Aprotinin 5 µg/ml Leupeptin 1% Triton x-100 1% Sodium deoxycholate 0.1% SDS 50 mM Tris, pH 7.4 NP-40 NaCl 150 mM NP-40 1% Tris, pH 8.0 50 mM HEPES Hepes pH 7.4 50mM NaCl 150mM Glycerol 10% Triton X-100 1% KCl 5mM EDTA […]

Odyssey infrared imaging system

Direct infrared fluorescence detection on the Odyssey Infrared Imaging System provides the established standard for Western blot analysis that can’t be equaled with chemiluminescence and visible fluorescence. Infrared detection gives you the quantitative analysis and wide linear dynamic range that chemiluminescence cannot. Strong and weak bands on the same blot are accurately detected without the […]

Spectrophotometric quantification of DNA and RNA

Because DNA and RNA absorb ultraviolet light, with a absorption peak at 260nm wavelength, spectrophotometers are commonly used to determine the concentration of DNA in a solution. Inside a spectrophotometer, a sample is exposed to ultraviolet light at 260 nm, and a photo-detector measures the light that passes through the sample. The more light absorbed […]