Comments on: 6X Laemmli buffer http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/ laboratory protocols and resources for your daily research Thu, 26 Jan 2012 21:58:36 +0000 hourly 1 http://wordpress.org/?v=3.2.1 By: Melody http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/comment-page-1/#comment-11270 Melody Thu, 26 Jan 2012 21:58:36 +0000 http://www.easyprotocols.com/blog2/?p=15#comment-11270 I need to make a 5X of SDS buffer. I currently have a protocol for 2x and if I add equal volume of dh20 to that I get a 1x. How do I calculate what I need to make the 5x solution. Can I multiply my 2x protocol by 3 to get a 5x stock solution? Your help would be greatly appreciated I need to make a 5X of SDS buffer. I currently have a protocol for 2x and if I add equal volume of dh20 to that I get a 1x. How do I calculate what I need to make the 5x solution.

Can I multiply my 2x protocol by 3 to get a 5x stock solution?

Your help would be greatly appreciated

]]> By: Theressa Schellin http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/comment-page-1/#comment-10855 Theressa Schellin Sat, 17 Dec 2011 16:41:41 +0000 http://www.easyprotocols.com/blog2/?p=15#comment-10855 2.) Can I just say what a relief to find someone who actually knows what theyre talking about on the internet. You definitely know how to bring an issue to light and make it important. More people need to read this and understand this side of the story. I cant believe youre not more <a href="http://www.cluehunt.com" rel="nofollow">popular </A>because you definitely have the gift. 2.) Can I just say what a relief to find someone who actually knows what theyre talking about on the internet. You definitely know how to bring an issue to light and make it important. More people need to read this and understand this side of the story. I cant believe youre not more popular because you definitely have the gift.

]]> By: jason http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/comment-page-1/#comment-10849 jason Sat, 17 Dec 2011 02:34:08 +0000 http://www.easyprotocols.com/blog2/?p=15#comment-10849 That is the appropriate blog for anybody who wants to seek out out about this topic. You realize so much its virtually hard to argue with you (not that I truly would need…HaHa). You definitely put a brand new spin on a topic thats been written about for years. Great stuff, simply great! That is the appropriate blog for anybody who wants to seek out out about this topic. You realize so much its virtually hard to argue with you (not that I truly would need…HaHa). You definitely put a brand new spin on a topic thats been written about for years. Great stuff, simply great!

]]> By: Anant http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/comment-page-1/#comment-7510 Anant Sun, 07 Aug 2011 09:37:49 +0000 http://www.easyprotocols.com/blog2/?p=15#comment-7510 Hi,Happy friendship day i want a total protein isolation protocol for wheat to carry out protein fingerprinting of various variety of wheat Hi,Happy friendship day i want a total protein isolation protocol for wheat to carry out protein fingerprinting of various variety of wheat

]]> By: Helen http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/comment-page-1/#comment-7497 Helen Sat, 06 Aug 2011 16:48:30 +0000 http://www.easyprotocols.com/blog2/?p=15#comment-7497 thanks thanks

]]> By: Naou http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/comment-page-1/#comment-5772 Naou Fri, 20 May 2011 10:24:16 +0000 http://www.easyprotocols.com/blog2/?p=15#comment-5772 Hy Everybody I would like to know, if i can use this buffer leamly 6X to do a total protein extraction from Nicotiana Bentamiana, tks Hy Everybody

I would like to know, if i can use this buffer leamly 6X to do a total protein extraction from Nicotiana Bentamiana,

tks

]]> By: Pete http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/comment-page-1/#comment-5603 Pete Mon, 09 May 2011 16:03:20 +0000 http://www.easyprotocols.com/blog2/?p=15#comment-5603 Hi, if u wanna substitute dtt for b-mercaptoethanol, is it still the same amount you listed? Thanks! Hi, if u wanna substitute dtt for b-mercaptoethanol, is it still the same amount you listed?

Thanks!

]]> By: Susan http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/comment-page-1/#comment-4553 Susan Mon, 14 Mar 2011 19:11:48 +0000 http://www.easyprotocols.com/blog2/?p=15#comment-4553 Why is there no 2-mercaptoethanol? Why is there no 2-mercaptoethanol?

]]> By: Azfer http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/comment-page-1/#comment-3968 Azfer Wed, 16 Feb 2011 15:15:04 +0000 http://www.easyprotocols.com/blog2/?p=15#comment-3968 Hi Can Laemmli sample buffer(2X) be used to lyse the cells in culture?If so, the bromophenol blue be added later after the OD is measured for protein concentration Hi
Can Laemmli sample buffer(2X) be used to lyse the cells in culture?If so, the bromophenol blue be added later after the OD is measured for protein concentration

]]> By: Nelson http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/comment-page-1/#comment-3055 Nelson Wed, 27 Oct 2010 00:13:57 +0000 http://www.easyprotocols.com/blog2/?p=15#comment-3055 hello everyone I would like to ask you wich is the range of DTT for denaturing condition. There are several concentration on internet. hello everyone

I would like to ask you wich is the range of DTT for denaturing condition. There are several concentration on internet.

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