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	<title>Easy protocols &#187; Buffers &amp; recipes</title>
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	<link>http://www.easyprotocols.com</link>
	<description>laboratory protocols and resources for your daily research</description>
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		<title>Most commonly used lysis buffers</title>
		<link>http://www.easyprotocols.com/most-commonly-used-lysis-buffers/</link>
		<comments>http://www.easyprotocols.com/most-commonly-used-lysis-buffers/#comments</comments>
		<pubDate>Thu, 04 Jun 2009 21:35:28 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Buffers & recipes]]></category>
		<category><![CDATA[lysate]]></category>
		<category><![CDATA[lysis buffer]]></category>
		<category><![CDATA[Western blot]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=135</guid>
		<description><![CDATA[RIPA

150 mM NaCl
1 mM PMSF
1 mM EDTA
5 µg/ml Aprotinin
5 µg/ml Leupeptin
1% Triton x-100
1% Sodium deoxycholate
0.1% SDS
50 mM Tris, pH 7.4

NP-40

NaCl 150 mM
NP-40 1%
Tris, pH 8.0 50 mM

HEPES

Hepes pH 7.4 50mM
NaCl 150mM
Glycerol 10%
Triton X-100 1%
KCl 5mM
EDTA 1mM
Protease inhibitors (leupeptin 10ug/ul, aprotinin 10ug/ul, PMSF 1mM)
Phosphatase inhibitors (NaF 50mM, Na3VO4 0.5mM, Na4P2O7 5mM)
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		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>10X TBS (Tris Buffered Saline)</title>
		<link>http://www.easyprotocols.com/10x-tbs-tris-buffered-saline/</link>
		<comments>http://www.easyprotocols.com/10x-tbs-tris-buffered-saline/#comments</comments>
		<pubDate>Tue, 12 May 2009 23:14:30 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Buffers & recipes]]></category>
		<category><![CDATA[TBS]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=121</guid>
		<description><![CDATA[To prepare 1 liter of 10X TBS:
30 gr  Tris
88 gr NaCl
2 gr KCl
mix everything in dwater until it&#8217;s disolved. Adjust pH at 7.5
]]></description>
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		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Paraformaldehide 4%</title>
		<link>http://www.easyprotocols.com/paraformaldehide-4/</link>
		<comments>http://www.easyprotocols.com/paraformaldehide-4/#comments</comments>
		<pubDate>Fri, 01 May 2009 20:39:24 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Buffers & recipes]]></category>
		<category><![CDATA[PFA fixation]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=112</guid>
		<description><![CDATA[To prepare 100ml of PFA 4% stock solution:
- Add 86ml ddwater in an enlermeyer
- Boil water in the microwave
- Weigh 4g of PFA
From now on in the fume hood
- Transfer PFA in the erlenmeyer
- Add a clean stirring bar
- Add a drop of NaOH 10M
- Stir it and keep it hot (heat at #1-2) till it goes [...]]]></description>
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		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Western blot protocol</title>
		<link>http://www.easyprotocols.com/western-blot-protocol/</link>
		<comments>http://www.easyprotocols.com/western-blot-protocol/#comments</comments>
		<pubDate>Mon, 06 Apr 2009 02:34:47 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Buffers & recipes]]></category>
		<category><![CDATA[Western blot]]></category>
		<category><![CDATA[cell lysates]]></category>
		<category><![CDATA[western blot buffers]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/blog2/?p=52</guid>
		<description><![CDATA[A complete western blot protocol from Howel lab in University of California San Diego. Here is the link:
http://cancer.ucsd.edu/howelllab/Western.html
From how to obtain a cell lysate to how to prepare the gels, running the gels, membrane transfer, detection and stripping the membrane. Also all the reagents and buffers you need for your western blot.
There is many techniques, this [...]]]></description>
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		<slash:comments>0</slash:comments>
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		<item>
		<title>6X Laemmli buffer</title>
		<link>http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/</link>
		<comments>http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/#comments</comments>
		<pubDate>Thu, 05 Mar 2009 22:22:53 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Buffers & recipes]]></category>
		<category><![CDATA[Western blot]]></category>
		<category><![CDATA[laemmli buffer]]></category>
		<category><![CDATA[SDS-PAGE]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/blog2/?p=15</guid>
		<description><![CDATA[Laemmli is a sample buffer to use in western blot.  6X sample buffer is added to each protein sample and is boiled or heated for 5-10 minutes.
Recipe to prepare 10 ml:
- 1.2gr SDS (sodium dodecyl sulfate)
- 6mg bromophenol blue
- 4.7ml glycerol
- 1.2ml Tris 0.5M pH6.8
- 2.1ml dwater
warm it a little bit and shake it till [...]]]></description>
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		<slash:comments>6</slash:comments>
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