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	<title>Easy protocols &#187; Cell culture</title>
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	<description>laboratory protocols and resources for your daily research</description>
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		<title>Protocol for Poly-D-Lysine Preparation and Plate Coating</title>
		<link>http://www.easyprotocols.com/protocol-for-poly-d-lysine-preparation-and-plate-coating/</link>
		<comments>http://www.easyprotocols.com/protocol-for-poly-d-lysine-preparation-and-plate-coating/#comments</comments>
		<pubDate>Wed, 13 May 2009 23:22:55 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Cell culture]]></category>
		<category><![CDATA[coating]]></category>
		<category><![CDATA[Poly-D-lysin]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=124</guid>
		<description><![CDATA[1. Prepare Poly-D-Lysine
a. Add 100 ml of sterile tissue culture grade H2O to 5 mg poly-D-lysine (Sigma P6407). Final concentration of poly-D-lysine is 50 ug/ml.
b. Mix by pipetting several times.
c. Store at 2-8 ºC or –20 ºC.
2. Prepare Coated Plates
a. Add enough poly-D-lysine solution to cover culture plate surface.
b. Incubate at room temperature for 1-24 [...]]]></description>
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		<title>Cell trypsinisation</title>
		<link>http://www.easyprotocols.com/cell-trypsinisation/</link>
		<comments>http://www.easyprotocols.com/cell-trypsinisation/#comments</comments>
		<pubDate>Sun, 05 Apr 2009 02:27:53 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Cell culture]]></category>
		<category><![CDATA[trypsin]]></category>
		<category><![CDATA[trypsinization]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/blog2/?p=48</guid>
		<description><![CDATA[1. Remove medium from culture dish.
2. Gently wash cells in a PBS without Ca or Mg.
3. Remove the wash solution.
4. Add trypsin-EDTA solution to cover the bottom of the culture dish (2ml for 10cm dish, 0.5ml for 60mm dish).
5. Put dish in the 37°C incubator for up to 5 minutes.
6. Check cells under microscope. Cells [...]]]></description>
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		<title>Heat inactivating serum</title>
		<link>http://www.easyprotocols.com/heat-inactivating-serum/</link>
		<comments>http://www.easyprotocols.com/heat-inactivating-serum/#comments</comments>
		<pubDate>Sat, 04 Apr 2009 02:24:46 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Cell culture]]></category>
		<category><![CDATA[heat inactivation]]></category>
		<category><![CDATA[serum]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/blog2/?p=44</guid>
		<description><![CDATA[1. Place bottles in a 56oC water bath. Make sure the bottles cannot tip over or otherwise become submerged in the water bath. The temperature is critical for complement degredation, so make sure!
2. Wait until the temperature stabilizes at 56oC (after adding the serum to the water bath)
3. Gently swirl the bottles every 10 minutes [...]]]></description>
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		<title>The basics about cell culture methods</title>
		<link>http://www.easyprotocols.com/the-basics-about-cell-culture-methods/</link>
		<comments>http://www.easyprotocols.com/the-basics-about-cell-culture-methods/#comments</comments>
		<pubDate>Fri, 03 Apr 2009 02:21:08 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Cell culture]]></category>
		<category><![CDATA[tissue culture]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/blog2/?p=41</guid>
		<description><![CDATA[The Department of Biological sciences of the University of Maryland (UMBC) has a protocol section very useful for cell culture first time users.
They explain extensively all the components of the work area and the equipment mainly used, from how the hood works to the microscope or the incubators. Also, types of cells that grow in [...]]]></description>
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		<title>Fundamental techniques in cell culture</title>
		<link>http://www.easyprotocols.com/fundamental-techniques-in-cell-culture/</link>
		<comments>http://www.easyprotocols.com/fundamental-techniques-in-cell-culture/#comments</comments>
		<pubDate>Thu, 02 Apr 2009 02:09:46 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Cell culture]]></category>
		<category><![CDATA[tissue culture]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/blog2/?p=36</guid>
		<description><![CDATA[Sigma-Aldrich publishes in its learning center web site a very complete guide to cell culture techniques.
It goes from the basic techniques to sterile techniques, freeze and thaw cells, culturing of semi-adherent or suspension cell lines, test for bacteria or fungi in the cultures, detection of mycoplasma,&#8230;
In summary, an extensive guide for cell culture beginners. Here [...]]]></description>
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