Fundamental techniques in cell culture

Sigma-Aldrich publishes in its learning center web site a very complete guide to cell culture techniques.
It goes from the basic techniques to sterile techniques, freeze and thaw cells, culturing of semi-adherent or suspension cell lines, test for bacteria or fungi in the cultures, detection of mycoplasma,…
In summary, an extensive guide for cell culture beginners. Here [...]

6X Laemmli buffer

Laemmli is a sample buffer to use in western blot.  6X sample buffer is added to each protein sample and is boiled or heated for 5-10 minutes.
Recipe to prepare 10 ml:
- 1.2gr SDS (sodium dodecyl sulfate)
- 6mg bromophenol blue
- 4.7ml glycerol
- 1.2ml Tris 0.5M pH6.8
- 2.1ml dwater
warm it a little bit and shake it till [...]