<?xml version="1.0" encoding="UTF-8"?>
<rss version="2.0"
	xmlns:content="http://purl.org/rss/1.0/modules/content/"
	xmlns:wfw="http://wellformedweb.org/CommentAPI/"
	xmlns:dc="http://purl.org/dc/elements/1.1/"
	xmlns:atom="http://www.w3.org/2005/Atom"
	xmlns:sy="http://purl.org/rss/1.0/modules/syndication/"
	xmlns:slash="http://purl.org/rss/1.0/modules/slash/"
	>

<channel>
	<title>Easy protocols &#187; PCR</title>
	<atom:link href="http://www.easyprotocols.com/category/molecular-biology/pcr/feed/" rel="self" type="application/rss+xml" />
	<link>http://www.easyprotocols.com</link>
	<description>laboratory protocols and resources for your daily research</description>
	<lastBuildDate>Wed, 16 Nov 2011 17:22:40 +0000</lastBuildDate>
	<language>en</language>
	<sy:updatePeriod>hourly</sy:updatePeriod>
	<sy:updateFrequency>1</sy:updateFrequency>
	<generator>http://wordpress.org/?v=3.2.1</generator>
		<item>
		<title>Good primer design tips</title>
		<link>http://www.easyprotocols.com/good-primer-design-tips/</link>
		<comments>http://www.easyprotocols.com/good-primer-design-tips/#comments</comments>
		<pubDate>Thu, 09 Apr 2009 05:45:48 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[PCR]]></category>
		<category><![CDATA[Molecular biology]]></category>
		<category><![CDATA[Primers]]></category>
		<category><![CDATA[Sequencing]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=72</guid>
		<description><![CDATA[1. Primers should be 17-24 bases in length 2. Base composition should be 45-65% (G+C) 3. Primers should end (3&#8242;) in a G or C, or CG or GC: this prevents &#8220;breathing&#8221; of ends and increases efficiency of priming 4. Tms between 55-75ºC are preferred 5. Primer self-complementarity (ability to form 2º structures such as [...]]]></description>
		<wfw:commentRss>http://www.easyprotocols.com/good-primer-design-tips/feed/</wfw:commentRss>
		<slash:comments>1</slash:comments>
		</item>
	</channel>
</rss>

