Cell trypsinisation

This post was written by admin on April 4, 2009
Posted Under: Cell culture

1. Remove medium from culture dish.
2. Gently wash cells in a PBS without Ca or Mg.
3. Remove the wash solution.
4. Add trypsin-EDTA solution to cover the bottom of the culture dish (2ml for 10cm dish, 0.5ml for 60mm dish).
5. Put dish in the 37°C incubator for up to 5 minutes.
6. Check cells under microscope. Cells are beginning to detach when they appear rounded.
7. When cells are in suspension, add growth medium supplemented with serum.
8. Dilute 1:2 up to 1:10.

Add a Comment

required, use real name
required, will not be published
optional, your blog address