Don’t throw the membranes away!

This post was written by admin on April 13, 2009
Posted Under: Western blot

Did you know that you can let western nitrocellulose and PVDF membranes dry up and keep them like this for a long time? Yes, you can ;)

I was doing that in my lab in Spain and when I came to US, I realized a lot of people was not doing it. When you are done with the western blot just wash the membrane with distilled water thoroughly to remove all ECL and put it on a tissue paper on your bench. Let it dry overnight and next day just put it in an envelope with the date and keep it in your notebook.

If you wanna use it later on, just add the blocking solution (or re-activate first with methanol in case of PVDF membranes) and it will re-hydrate. From here, proceed as usual. The proteins will still be there!

Actually, a friend of mine told me the other day that she has used membranes that she kept for as long as two years. And they worked!

So, you know now, recycling is good. You never know when you’re gonna need those samples again. Keep them just in case.

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