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	<title>Easy protocols</title>
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	<link>http://www.easyprotocols.com</link>
	<description>laboratory protocols and resources for your daily research</description>
	<lastBuildDate>Sat, 19 Sep 2009 01:56:24 +0000</lastBuildDate>
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		<title>Fast solidification of agarose gels</title>
		<link>http://www.easyprotocols.com/fast-solidification-of-agarose-gels/</link>
		<comments>http://www.easyprotocols.com/fast-solidification-of-agarose-gels/#comments</comments>
		<pubDate>Sat, 19 Sep 2009 01:56:24 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Tricks & short cuts]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=175</guid>
		<description><![CDATA[Hi there! Here is a simple but efficient trick to get your agarose gels to solidify much faster.  You have to weight the required amount of agarose as usual, but here it comes the trick. To dissolve this agarose, only pour HALF the amount of the required TAE 1% and save the rest (for example, to [...]]]></description>
		<wfw:commentRss>http://www.easyprotocols.com/fast-solidification-of-agarose-gels/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>You know you&#8217;ve worked too long in a lab when</title>
		<link>http://www.easyprotocols.com/you-know-youve-worked-too-long-in-a-lab-when/</link>
		<comments>http://www.easyprotocols.com/you-know-youve-worked-too-long-in-a-lab-when/#comments</comments>
		<pubDate>Fri, 26 Jun 2009 00:33:31 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[General]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=117</guid>
		<description><![CDATA[Very funny! and very true! hehehe. I got it from a friend in facebook. There is this facebook group called You know you&#8217;ve worked too long in a lab when, Daniel Sutton from London is the admin, but I don&#8217;t really know who is/are the author/authors of these hilarious statements. LOL
1. You wonder what absolute alcohol [...]]]></description>
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		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Saving time and money doing maxi/midi preps</title>
		<link>http://www.easyprotocols.com/saving-time-and-money-doing-maximidi-preps/</link>
		<comments>http://www.easyprotocols.com/saving-time-and-money-doing-maximidi-preps/#comments</comments>
		<pubDate>Mon, 22 Jun 2009 01:24:00 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Plasmid purification]]></category>
		<category><![CDATA[Tricks & short cuts]]></category>
		<category><![CDATA[maxiprep]]></category>
		<category><![CDATA[midiprep]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=152</guid>
		<description><![CDATA[Here is a trick. It is very simple, only a bit messy. But it will save you one centrifugation step and 30 minutes.
You have to pellet your overnight culture as usual, ressuspend it, lysate it and stop the lysis. So far, as recommended in the protocol. The next step is normally a centrifugation to clear [...]]]></description>
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		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>George W. Bush contribution to science</title>
		<link>http://www.easyprotocols.com/george-w-bush-contribution-to-science/</link>
		<comments>http://www.easyprotocols.com/george-w-bush-contribution-to-science/#comments</comments>
		<pubDate>Mon, 15 Jun 2009 00:20:02 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[General]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=148</guid>
		<description><![CDATA[Ex-president George W. Bush inesperately contributed to science when he was able to duck flying shoes thrown at him by an Iraqi reporter in December in Bagdad as reveals the study published this month in the magazine Current Biology by two neurologists of Washington University.
Bush reflexes and the impassivity that al-Maliki, the Iraqi primer Minister, [...]]]></description>
		<wfw:commentRss>http://www.easyprotocols.com/george-w-bush-contribution-to-science/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Most commonly used lysis buffers</title>
		<link>http://www.easyprotocols.com/most-commonly-used-lysis-buffers/</link>
		<comments>http://www.easyprotocols.com/most-commonly-used-lysis-buffers/#comments</comments>
		<pubDate>Thu, 04 Jun 2009 21:35:28 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Buffers & recipes]]></category>
		<category><![CDATA[lysate]]></category>
		<category><![CDATA[lysis buffer]]></category>
		<category><![CDATA[Western blot]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=135</guid>
		<description><![CDATA[RIPA

150 mM NaCl
1 mM PMSF
1 mM EDTA
5 µg/ml Aprotinin
5 µg/ml Leupeptin
1% Triton x-100
1% Sodium deoxycholate
0.1% SDS
50 mM Tris, pH 7.4

NP-40

NaCl 150 mM
NP-40 1%
Tris, pH 8.0 50 mM

HEPES

Hepes pH 7.4 50mM
NaCl 150mM
Glycerol 10%
Triton X-100 1%
KCl 5mM
EDTA 1mM
Protease inhibitors (leupeptin 10ug/ul, aprotinin 10ug/ul, PMSF 1mM)
Phosphatase inhibitors (NaF 50mM, Na3VO4 0.5mM, Na4P2O7 5mM)
]]></description>
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		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Odyssey infrared imaging system</title>
		<link>http://www.easyprotocols.com/odyssey-infrared-imaging-system/</link>
		<comments>http://www.easyprotocols.com/odyssey-infrared-imaging-system/#comments</comments>
		<pubDate>Wed, 03 Jun 2009 22:26:47 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Western blot]]></category>
		<category><![CDATA[odyssey]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=138</guid>
		<description><![CDATA[Direct infrared fluorescence detection on the Odyssey Infrared Imaging System provides the established standard for Western blot analysis that can&#8217;t be equaled with chemiluminescence and visible fluorescence. Infrared detection gives you the quantitative analysis and wide linear dynamic range that chemiluminescence cannot. Strong and weak bands on the same blot are accurately detected without the [...]]]></description>
		<wfw:commentRss>http://www.easyprotocols.com/odyssey-infrared-imaging-system/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Spectrophotometric quantification of DNA and RNA</title>
		<link>http://www.easyprotocols.com/spectrophotometric-quantification-of-dna-and-rna/</link>
		<comments>http://www.easyprotocols.com/spectrophotometric-quantification-of-dna-and-rna/#comments</comments>
		<pubDate>Wed, 03 Jun 2009 01:13:54 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[General]]></category>
		<category><![CDATA[quantification absorvance]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=128</guid>
		<description><![CDATA[Because DNA and RNA absorb ultraviolet light, with a absorption peak at 260nm wavelength, spectrophotometers are commonly used to determine the concentration of DNA in a solution. Inside a spectrophotometer, a sample is exposed to ultraviolet light at 260 nm, and a photo-detector measures the light that passes through the sample. The more light absorbed [...]]]></description>
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		<slash:comments>1</slash:comments>
		</item>
		<item>
		<title>Protocol for Poly-D-Lysine Preparation and Plate Coating</title>
		<link>http://www.easyprotocols.com/protocol-for-poly-d-lysine-preparation-and-plate-coating/</link>
		<comments>http://www.easyprotocols.com/protocol-for-poly-d-lysine-preparation-and-plate-coating/#comments</comments>
		<pubDate>Wed, 13 May 2009 23:22:55 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Cell culture]]></category>
		<category><![CDATA[coating]]></category>
		<category><![CDATA[Poly-D-lysin]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=124</guid>
		<description><![CDATA[1. Prepare Poly-D-Lysine
a. Add 100 ml of sterile tissue culture grade H2O to 5 mg poly-D-lysine (Sigma P6407). Final concentration of poly-D-lysine is 50 ug/ml.
b. Mix by pipetting several times.
c. Store at 2-8 ºC or –20 ºC.
2. Prepare Coated Plates
a. Add enough poly-D-lysine solution to cover culture plate surface.
b. Incubate at room temperature for 1-24 [...]]]></description>
		<wfw:commentRss>http://www.easyprotocols.com/protocol-for-poly-d-lysine-preparation-and-plate-coating/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>10X TBS (Tris Buffered Saline)</title>
		<link>http://www.easyprotocols.com/10x-tbs-tris-buffered-saline/</link>
		<comments>http://www.easyprotocols.com/10x-tbs-tris-buffered-saline/#comments</comments>
		<pubDate>Tue, 12 May 2009 23:14:30 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Buffers & recipes]]></category>
		<category><![CDATA[TBS]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=121</guid>
		<description><![CDATA[To prepare 1 liter of 10X TBS:
30 gr  Tris
88 gr NaCl
2 gr KCl
mix everything in dwater until it&#8217;s disolved. Adjust pH at 7.5
]]></description>
		<wfw:commentRss>http://www.easyprotocols.com/10x-tbs-tris-buffered-saline/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Paraformaldehide 4%</title>
		<link>http://www.easyprotocols.com/paraformaldehide-4/</link>
		<comments>http://www.easyprotocols.com/paraformaldehide-4/#comments</comments>
		<pubDate>Fri, 01 May 2009 20:39:24 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Buffers & recipes]]></category>
		<category><![CDATA[PFA fixation]]></category>

		<guid isPermaLink="false">http://www.easyprotocols.com/?p=112</guid>
		<description><![CDATA[To prepare 100ml of PFA 4% stock solution:
- Add 86ml ddwater in an enlermeyer
- Boil water in the microwave
- Weigh 4g of PFA
From now on in the fume hood
- Transfer PFA in the erlenmeyer
- Add a clean stirring bar
- Add a drop of NaOH 10M
- Stir it and keep it hot (heat at #1-2) till it goes [...]]]></description>
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		<slash:comments>0</slash:comments>
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