Fundamental techniques in cell culture
Sigma-Aldrich publishes in its learning center web site a very complete guide to cell culture techniques.
It goes from the basic techniques to sterile techniques, freeze and thaw cells, culturing of semi-adherent or suspension cell lines, test for bacteria or fungi in the cultures, detection of mycoplasma,…
In summary, an extensive guide for cell culture beginners. Here is the link:
Basic Techniques, Aseptic Technique and Good Cell Culture Practice, Ressuscitation of Frozen Cell Lines, Subculture of Adherent Cell Lines, Subculture of Semi-Adherent Cell Lines, Subculture of Suspension Cell Lines, Cell Quantification, Cryopreservation of Cell Lines, Testing for Bacteria and Fungi, Detection of Mycoplasma by Culture, Testing for Mycoplasma by Indirect DNA Stain.