Paraformaldehide 4%

This post was written by admin on May 1, 2009
Posted Under: Buffers & recipes

To prepare 100ml of PFA 4% stock solution:

- Add 86ml ddwater in an enlermeyer
- Boil water in the microwave
- Weigh 4g of PFA

From now on in the fume hood
- Transfer PFA in the erlenmeyer
- Add a clean stirring bar
- Add a drop of NaOH 10M
- Stir it and keep it hot (heat at #1-2) till it goes clear (DO NOT LET PFA BOIL)
- Place on ice to cool it down
- Adjust volume to 90ml
- Add 10ml PBS 10X
- Adjust pH to 7.4

Store at RT or 4C

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