Cell culture and cell preparation

Fundamental Techniques in Cell Culture
Basic Techniques, Aseptic Technique and Good Cell Culture Practice, Ressuscitation of Frozen Cell Lines, Subculture of Adherent Cell
Lines, Subculture of Semi-Adherent Cell Lines, Subculture of Suspension Cell Lines, Cell Quantification, Cryopreservation of Cell
Lines, Testing for Bacteria and Fungi, Detection of Mycoplasma by Culture, Testing for Mycoplasma by Indirect DNA Stain. 

http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture...

Basics about Tissue Culture Methods
Types of cells grown in culture, work area and equipment, preservation and storage, maintenance, safety considerations, tissue culture
procedures.
http://www.research.umbc.edu/~jwolf/method5.htm

Single cell cloning by serial dilution
This technique is widely used for clonal isolation of hybridomas and other cell lines that are not attachment dependent. However, it is
also very useful for cloning attachment dependent cells when the cell plating efficiency is very low, unknown or unpredictable. This
method is fast and easy.
http://www.corning.com/Lifesciences/technical_information/techDocs/Sin...

PC12 cells
Growth of PC12 cells, poly-L-lysine coating of plates for PC12 secretion and transfections, harvesting PC12 cells for reporter assay,
Harvesting PC12 cells for reporter assay, Harvesting PC12 cells for reporter assay, Transfection of PC12 cells (Lipofectamine),
Transfection of PC12 cells (Superfect), Transfection of PC12 cells (Lipofectin), Transfection of PC12 cells (GenePorter).

http://medicine.ucsd.edu/hypertension/protocols.htm

Heat Inactivating Serum
http://www.easyprotocols.com/showprotocol.php?prot=48

Cell Trypsinisation
http://www.easyprotocols.com/showprotocol.php?prot=52