Cloning

Easy subcloning
Subcloning should be easy and fast, and work every time. The following protocols minimize the number of manipulations required to
prepare DNA fragments for ligations, thereby both saving time and increasing reliability.
http://info.med.yale.edu/mbb/koelle/

Dna ligation
Abstract, materials, procedure, critical steps, troubleshooting,...
http://openwetware.org/wiki/DNA_Ligation