General recipes and protocols

Histology lab protocols
More than one hundred histology protocols from University of Nottingham Medical School Division of Histopathology. See link below.

   10% FORMAL SALINE
   3-AMINOPROPYLTRIETHOXYSILANE TREATED SLIDES  
   4% PARAFORMALDEHYDE  
   ACETYL CHOLINESTERASE  
   ACID PHOSPHATASE  
   ACID PICRO MALLORY  
   ADENOSINE TRIPHOSPHATASE (ATPASE)  
   ALCIAN BLUE TECHNIQUE  
   ALKALINE CONGO RED TECHNIQUE  
   ALKALINE PHOSPHATASE (APAAP) TECHNIQUE  
   ALKALINE PHOSPHATASE  
   BIELSCHOWSKY METHOD  
   CARAZZI'S HAEMATOXYLIN  
   CELESTINE BLUE SOLUTION  
   CHROME GELATINISED SLIDES AND COVERSLIPS  
   COMBINED ALCIAN BLUE - P.A.S  
   CRESYL FAST VIOLET TECHNIQUE FOR NISSL  
   CRESYL FAST VIOLET  
   CROSS-PALMGRENS METHOD  
   CYTOCHROME OXIDASE TECHNIQUE  
   DECALCIFICATION  
   E.P.S.R.  
   ELASTIC VAN GIESON (EVG)  
   FEULGEN REACTION FOR DNA  
   FORMALDEHYDE PRECAUTIONS  
   FOUCHET METHOD  
   GALLYAS TECHNIQUE  
   GIEMSA FOR Helicobacter pylori  
   GILLS HAEMATOXYLIN  
   GIMENEZ STAIN  
   GLUTARALDEHYDE  
   GOMORI TRICHROME  
   GORDON AND SWEETS RETICULIN METHOD  
   GRAM STAIN  
   GRIMELIUS  
   HAEMATOXYLIN AND EOSIN TECHNIQUE  
   HAEMATOXYLIN VAN GIESON  
   HAGA-YAMAGCHI METHENAMINE SILVER TECHNIQUE  
   HARRIS'S HAEMATOXYLIN  
   HIGH IRON DIAMINE  
   HIGHMANS CONGO RED  
   INDIRECT PEROXIDASE TECHNIQUE Fixed tissue  
   INDIRECT PEROXIDASE TECHNIQUE  
   JONES METHENAMINE SILVER  
   LACTASE  
   LEUCINE-AMINO PEPTIDASE  
   M.S.B TECHNIQUE  
   MASSON FONTANA  
   MASSON FONTANA  
   MASSONS TRICHOME  
   MAY GRUNWALD - GIEMSA  
   MELANIN BLEACH  
   METHENAMINE SILVER FOR FUNGI  
   METHYL GREEN PYRONIN  
   MILLERS ELASTIC STAIN  
   Mount sections in aqueous mountant  
   Mount sections in DPX  
   MYODENYLATE DEAMINASE  
   NADH DIAPHORASE  
   NON-SPECIFIC ESTERASE  
   OIL RED O  
   PAPANICOLAOU METHOD  
   PERIODIC ACID SCHIFFS ORANGE G  
   PERIODIC ACID, SCHIFFS  
   PERLS TECHNIQUE  
   PEROXIDASE - ANTI - PEROXIDASE TECHNIQUE  
   PHLOXINE TARTRAZINE  
   PHOSPHORYLASE  
   PHOSPHOTUNGSTIC ACID HAEMATOXYLIN  
   POLY-L-LYSINE COATED SLIDES  
   REMOVAL OF FORMALIN PIGMENT  
   RESIN EMBEDDING FOR HIGH RESOLUTION MORPHOLOGY  
   RESIN PROCESSING OF BONE  
   RESIN STAINING METHODS  
   RUBEANIC ACID TECHNIQUE  
   SCHMORL TECHNIQUE  
   SECTIONING RESIN EMBEDDED TISSUE  
   SHIKATA TECHNIQUE  
   SOLOCHROME CYANIN TECHNIQUE  
   SOUTHGATES MUCICARMINE  
   STAIN FOR ACID FAST BACILLI  
   STAINING RESIN SECTIONS OF BONE  
   SUCCINATE DEHYDROGENASE  
   SUCRASE  
   THE AVIDIN/BIOTIN (ABC) TECHNIQUE  
   TOLUIDINE BLUE IN SORENSONS pH 6.8 BUFFER  
   TOLUIDINE BLUE  
   TRYPSIN DIGESTION  
   VAN GIESON  
   VON KOSSA  
   WARTHIN - STARRY  
   WEIL'S MYELIN STAIN


http://www.nottingham.ac.uk/pathology/default.html

Sectioning

Frozen section technique
A method for preparation of frozen sections
http://pathologyinnovations.com/frozen_section_technique.htm

Staining

Staining Procedures
Acid Fast Stain (for mycobacteria)
Acid Fast Stain (Ziehl-Neelsen, for M. leprae and Nocardia) [contributed by Greg Winn, Specialist Histopathology & Cytology Services,
Western Australia]
Fite's Acid Fast Stain
Alcian Blue Stain
Alcian Blue-PAS Stain (PAB)
Hyaluronidase Digestion for Alcian Blue
Alizarin Stain for calcium
Auramine-Rhodamine Stain (fluorescent)
Bielschowsky Stain (for senile plaques)
Bile Stain
Bodian's Stain
Colloidal Iron Stain
Congo Red Stain
Copper Stain
Elastic van Gieson Stain
Elastic - Weigert's resorcin-fuchsin method
Modified Elastic van Gieson Stain
Fontana-Masson Stain for melanin
Melanin Bleach
Fraser Lendrum Stain
Giemsa (Modified May-Gruenwald) Stain (for hematopoietic tissues)
Giemsa Stain (for Helicobacter)
Gram (Modified Brown-Brenn) Stain
Gridley's Stain for ameba
Grimelius Argyrophil Stain (Pascual's Method)
Grocott's Methenamine Silver (GMS) Stain
Holzer's Glial Fiber Stain
Hortega's Pineal Stain
Iron Stain (Prussian blue)
Iron Stain (Turnbull's blue)
Jones' Silver Stain
Luxol Fast Blue (LFB) Stain
Methyl Green Pyronin (MGP) Stain
Mucicarmine Stain
Nissl Stain
Oil Red O Stain
Orcein Stain
Periodic acid-Schiff Stain (PAS)
Periodic acid-Schiff, digested Stain (PAS-D)
PTAH Stain
Reticulin Stain
Spirochete Stain (Steiner & Steiner method)
Sudan Black B Stain (for lipochrome)
Sudan Black B Stain (for fat)
Trichrome Stain - Masson's method
Trichrome Stain - microwave method
Thioflavin S Stain (for amyloid in tissues)
Modified Thioflavin S Stain (for senile plaques)
Toluidine Blue Stain (for mast cells)
Urate Crystal Stain
VonKossa Stain for calcium



http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MANUALS.html

General recipes and protocols

Methods and Techniques for histologists
Histology protocols from other online resources

Solutions and Buffers (Gerard Lazo Lab) 
Frozen Section Technique - A Method for Preparation of Frozen Sections (Pathology Innovations)
Special Stains in Histology (The Internet Pathology Laboratory)
Special Staining Protocols (StainsFile)
Laboratory Protocols (University of Bristol Veterinary Pathology) 
Histotechnology Technical Methods (University of Nottingham Pathology)
Histotechniques (The Internet Pathology Laboratory)
Histology Protocols (ProtocolOnline)
Histology Protocols (BioVisa) 
Beta-Gal Staining- Whole Mount Embryos (Karl J Clark)
Detection of ß-Galactosidase and Alkaline Phosphatase Activities in Tissue (Harvard Medical)
Embedding and Sectioning of Embryos
Michelson Lab Protocols
http://www.ihcworld.com/protocols.htm