Immunoprecipitation

General Principles of Immunoprecipitation
The choice of a lysis buffer (RIPA buffer, NP40 buffer, PBS, TN).
Immunoprecipitation (Boiling in SD, Antisera, Bugs, Secondary Antibodies, Cell Lysis, Pre-clearing).

http://pingu.salk.edu/~sefton/Hyper_protocols/immunoprecip.html