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C. elegans Caenorhabditis elegans server
WormBase: Genome Blast/Blat, WormMart, Batch, Sequences, Markers, Genetic Maps, Literature search,...
Recent papers, Meetings, CGC: Caenorhabditis Genetics Center, Labs & Researchers, Wormatlas, Software, Nematodes, The Genome, Movies,
WormBook.
http://elegans.swmed.edu/
Drosophila Virtual library: Drosophila
This directory points to internet resources for research on the fruit fly Drosophila melanogaster. What is Drosophila? Drosophila
genome project, Drosophila labs on the web, the interactive fly, Drosophila neurobiology, fly stock centers, PROTOCOLS, sequence and
genome resources, comercial suppliers.
http://www.ceolas.org/fly/
Yeast Yeast protocols from Herskowitz lab
YEAST GENETIC PROTOCOLS (Yeast Transformation, Sleazy Plate Transformation of Yeast, PCR to check genotype, Bgal filter assay, Bgal
plate overlay assay, Bgal liquid assay, Liquid Sporulation, EMS Mutagenesis, Generating a Temperature Sensitive Allele, Yale Genome
Analysis Center, Useful Templates for Yeast Genetics, Sequences). YEAST CELL BIOLOGY (Immunofluorescence for Epitope Tagged Proteins,
Dapi Staining, FACS Analysis). YEAST BIOCHEMISTRY (Extracts for Yeast Westerns, Silver Staining Protocol (Diamine Staining)).
http://biochemistry.ucsf.edu/%7Eherskowitz/protocols.html
The Gietz Lab Yeast Transformation Home Page
The BEST method, 2 Hybrid method, Quick & Easy method, Frozen method, YEAST Media, Solutions and F.A.Q.
http://www.umanitoba.ca/faculties/medicine/biochem/gietz/Trafo.html
Xenopus Xenopus oocytes isolation
Materials, oocyte removal, defolliculation, selecting good oocytes, plating oocytes, washing oocytes.
http://www.serva.de/products/sheets/Prot_Xenopus_oocyte.pdf
Xenopus oocyte isolation, maturation, enucleation, fractionation & injection.
http://spot.colorado.edu/~klym/Methods/oocyteIsolation.htm
Isolation of Oocytes from Xenopus lavis
http://research.musc.edu/dlar/Isolation%20of%20Oocytes.htm
Micro-injection of oocytes and embryos from Xenopus
Oocyte MICRO-INJECTION: Preparation (Glass capillary needles, Petri dishes, Stock solutions, Modified Barths Media, Operating
equipment, Collection of oocytes), On The Day (Preparation of oocytes, Setting up the needle, Injection, RNA extraction). Embryo
MICRO-INJECTION: Embryo Injection, Embryo labelling, BrdU, Embryo Histology, Whole Mount in situ hybridisation, Wax Embedding,
Rehydration of sections, Cryostat Sectioning & Non-isotopic in situ hybridisation to sectioned material, Cryostat Sectioning &
Non-isotopic Fluorescence staining of sectioned material, Membrane staining with soybean agglutinin, Hoechst Staining of nuclei.
http://www.chuq.qc.ca/labomoss/Protocols/Protocols%20X%20ooc%20emb.htm...
Mouse and rat Trangenic mouse breeding
Introduction, Breeding Suggestions, ES Cell-Mouse Chimera Breeding, Record Keeping, Transmission Patterns, Vital Statistics, Sources of
Additional Information, The NIH Guide for the Care and Use of Laboratory Animals is available online.
http://www.med.umich.edu/tamc/breed.html
Transgenic outline: mouse and rats
Introduction, Experimental Timeline, Plan the Experiment, Clone the Transgene
Transgene Screening, Transgene Expression, DNA Microinjection, Identify Transgenic Founders, Southern Analysis, Transgenic Analysis,
Pertinent Publications, Review Articles and Books.
http://www.med.umich.edu/tamc/tgoutline.html
Tail DNA for PCR (No Organic Solvents)
Use mouse tail dna directly for PCR after proteinase K digestion.
http://www.jax.org/imr/tail_nonorg.html
DNA from Tail Biopsies
DNA from Tail Biopsies, Mouse tail clipping DNA genotyping.
http://www.jax.org/imr/tail_phenol.html
Mouse genome informatics
Mouse Genome Informatics (MGI) provides integrated access to data on the genetics, genomics, and biology of the laboratory mouse.
http://www.informatics.jax.org/
Universal mouse genotyping protocol using PCR
Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core.
http://mgc.wustl.edu/protocols/pcr_genotyping.html
Arabidopsis thaliana The Arabidopsis Information Resource (TAIR)
The Arabidopsis Information Resource (TAIR) maintains a database of genetic and molecular biology data for the model higher plant
Arabidopsis thaliana. Data available from TAIR includes the complete genome sequence along with gene structure, gene product
information, metabolism, gene expression, DNA and seed stocks, genome maps, genetic and physical markers, publications, and
information about the Arabidopsis research community
http://www.arabidopsis.org/
Zebrafish Basic zebrafish protocols
Isolation of embryos.
Wholemount antibody staining.
http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Fish/fish_basic.html
ZFIN: The Zebrafish Model Organism Database
Genes, markers, clones, expression, BLAST, mutants, anatomy, maps, publications.
http://zfin.org/cgi-bin/webdriver?MIval=aa-ZDB_home.apg
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