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Mouse and rat Trangenic mouse breeding Introduction, Breeding Suggestions, ES Cell-Mouse Chimera Breeding, Record Keeping, Transmission Patterns, Vital Statistics, Sources of
Additional Information, The NIH Guide for the Care and Use of Laboratory Animals is available online.
http://www.med.umich.edu/tamc/breed.html
Transgenic outline: mouse and rats Introduction, Experimental Timeline, Plan the Experiment, Clone the Transgene
Transgene Screening, Transgene Expression, DNA Microinjection, Identify Transgenic Founders, Southern Analysis, Transgenic Analysis,
Pertinent Publications, Review Articles and Books. http://www.med.umich.edu/tamc/tgoutline.html
Tail DNA for PCR (No Organic Solvents) Use mouse tail dna directly for PCR after proteinase K digestion. http://www.jax.org/imr/tail_nonorg.html
DNA from Tail Biopsies DNA from Tail Biopsies, Mouse tail clipping DNA genotyping. http://www.jax.org/imr/tail_phenol.html
Mouse genome informatics Mouse Genome Informatics (MGI) provides integrated access to data on the genetics, genomics, and biology of the laboratory mouse. http://www.informatics.jax.org/
Universal mouse genotyping protocol using PCR Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core. http://mgc.wustl.edu/protocols/pcr_genotyping.html |