MODEL ORGANISMS PROTOCOLS & RESOURCES

Mouse and rat

Trangenic mouse breeding
Introduction, Breeding Suggestions, ES Cell-Mouse Chimera Breeding, Record Keeping, Transmission Patterns, Vital Statistics, Sources of Additional Information, The NIH Guide for the Care and Use of Laboratory Animals is available online.
http://www.med.umich.edu/tamc/breed.html

Transgenic outline: mouse and rats
Introduction, Experimental Timeline, Plan the Experiment, Clone the Transgene Transgene Screening, Transgene Expression, DNA Microinjection, Identify Transgenic Founders, Southern Analysis, Transgenic Analysis, Pertinent Publications, Review Articles and Books.
http://www.med.umich.edu/tamc/tgoutline.html

Tail DNA for PCR (No Organic Solvents)
Use mouse tail dna directly for PCR after proteinase K digestion.
http://www.jax.org/imr/tail_nonorg.html

DNA from Tail Biopsies
DNA from Tail Biopsies, Mouse tail clipping DNA genotyping.
http://www.jax.org/imr/tail_phenol.html

Mouse genome informatics
Mouse Genome Informatics (MGI) provides integrated access to data on the genetics, genomics, and biology of the laboratory mouse.
http://www.informatics.jax.org/

Universal mouse genotyping protocol using PCR
Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core.
http://mgc.wustl.edu/protocols/pcr_genotyping.html