PCR

Protocol for PCR with Taq DNA Polymerase
Avoiding Contamination, Preparation of Reaction Mixture, Components of the Reaction Mixture, Cycling Conditions.
http://www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm

General Notes on Primer Design in PCR 
Perhaps the most critical parameter for successful PCR is the design of Primers. All things being equal, a poorly designed primer can
result in a PCR reaction that will not work. The primer sequence determines several things such as the length of the product, its
melting temperature and ultimately the yield. A poorly designed primer can result in little or no product due to non-specific
amplification and/or primer-dimer formation, which can become competitive enough to suppress product formation. This application note
is provided to give rules that should be taken into account when designing primers for PCR. More comprehensive coverage of this
subject can be found elsewhere.
http://www.labprotocol.com/detail.php?siteid=52