Sequencing

Recombinant Dna isolation, cloning and sequencing
Bst-catalyzed radiolabeled DNA sequencing, Radiolabeled sequencing gel preparation, loading, and electrophoresis, Taq-polymerase
catalyzed cycle sequencing using fluorescent-labeled dye primers, Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled
dye terminator reactions, Terminator Reaction Clean-Up via Centri-Sep Columns, Terminator Reaction Clean-Up via Sephadex G-50 Filled
Microtiter Format Filter Plates, Sequenase[TM] catalyzed sequencing with dye-labeled terminators, Fluorescent-labeled sequencing gel
preparation, pre-electrophoresis, sample loading, electrophoresis, data collection, and analysis on the ABI 373A DNA sequencer,
Double-stranded sequencing of cDNA clones containing long poly(A) tails using anchored poly(dT) primers, cDNA sequencing based on PCR
and random shotgun cloning.

http://www.genome.ou.edu/protocol_book/protocol_index.html

Sequencing protocol (Big Dye Terminator) 
Amplify the gene, purify tha sequencing template, run the sequencing reaction, purify the sequencing product, dry/submit the sample.

http://depts.washington.edu/bakerpg/protocols/sequencing_protocol.html