Western blot

Western protocol
Detailed western protocol and buffers preparation.
http://cancer.ucsd.edu/howelllab/Western.html

All about western blot
Protocols, troubleshooting, theory, buffers and definitions.
http://www.westernblotting.org/

Western blot troubleshooting
High background signal, strange/non-specific bands on the blot, low sensitivity, is a right band detected?, uneven results with lots of
spots.

http://www.agrisera.se/protocols/westerntrouble.shtml