MODEL ORGANISMS PROTOCOLS & RESOURCES

Xenopus

Xenopus oocytes isolation
Materials, oocyte removal, defolliculation, selecting good oocytes, plating oocytes, washing oocytes.
http://www.serva.de/products/sheets/Prot_Xenopus_oocyte.pdf

Xenopus oocyte isolation, maturation, enucleation, fractionation & injection.

http://spot.colorado.edu/~klym/Methods/oocyteIsolation.htm

Isolation of Oocytes from Xenopus lavis

http://research.musc.edu/dlar/Isolation%20of%20Oocytes.htm

Micro-injection of oocytes and embryos from Xenopus
Oocyte MICRO-INJECTION: Preparation (Glass capillary needles, Petri dishes, Stock solutions, Modified Barths Media, Operating equipment, Collection of oocytes), On The Day (Preparation of oocytes, Setting up the needle, Injection, RNA extraction). Embryo MICRO-INJECTION: Embryo Injection, Embryo labelling, BrdU, Embryo Histology, Whole Mount in situ hybridisation, Wax Embedding, Rehydration of sections, Cryostat Sectioning & Non-isotopic in situ hybridisation to sectioned material, Cryostat Sectioning & Non-isotopic Fluorescence staining of sectioned material, Membrane staining with soybean agglutinin, Hoechst Staining of nuclei.
http://www.chuq.qc.ca/labomoss/Protocols/Protocols%20X%20ooc%20emb.htm...