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	<title>Easy protocols &#187; laemmli buffer</title>
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		<title>6X Laemmli buffer</title>
		<link>http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/</link>
		<comments>http://www.easyprotocols.com/6x-laemmli-buffer-sample-buffer-for-western-blot/#comments</comments>
		<pubDate>Thu, 05 Mar 2009 22:22:53 +0000</pubDate>
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				<category><![CDATA[Buffers & recipes]]></category>
		<category><![CDATA[Western blot]]></category>
		<category><![CDATA[laemmli buffer]]></category>
		<category><![CDATA[SDS-PAGE]]></category>

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		<description><![CDATA[Laemmli is a sample buffer to use in western blot.  6X sample buffer is added to each protein sample and is boiled or heated for 5-10 minutes. Recipe to prepare 10 ml: - 1.2gr SDS (sodium dodecyl sulfate) - 6mg bromophenol blue - 4.7ml glycerol - 1.2ml Tris 0.5M pH6.8 - 2.1ml dwater warm it [...]]]></description>
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