Most commonly used lysis buffers
RIPA 150 mM NaCl 1 mM PMSF 1 mM EDTA 5 µg/ml Aprotinin 5 µg/ml Leupeptin 1% Triton x-100 1% Sodium deoxycholate 0.1% SDS 50 mM Tris, pH 7.4 NP-40 NaCl 150 mM NP-40 1% Tris, pH 8.0 50 mM HEPES Hepes pH 7.4 50mM NaCl 150mM Glycerol 10% Triton X-100 1% KCl 5mM EDTA [...]