RIPA 150 mM NaCl 1 mM PMSF 1 mM EDTA 5 µg/ml Aprotinin 5 µg/ml Leupeptin 1% Triton x-100 1% Sodium deoxycholate 0.1% SDS 50 mM Tris, pH 7.4 NP-40 NaCl 150 mM NP-40 1% Tris, pH 8.0 50 mM HEPES Hepes pH 7.4 50mM NaCl 150mM Glycerol 10% Triton X-100 1% KCl 5mM EDTA [...]
Everything you always wanted to learn about western blots but were too afraid to ask is in this web site: http://www.westernblotting.org/ Western blot protocols, troubleshoting, definitions, buffers and theory all in a web site. What is biotin? how the stripping buffer works? how do I prepare the lysis buffer? In a very basic way, you’ll find your answers in this [...]