Most commonly used lysis buffers

RIPA

150 mM NaCl
1 mM PMSF
1 mM EDTA
5 µg/ml Aprotinin
5 µg/ml Leupeptin
1% Triton x-100
1% Sodium deoxycholate
0.1% SDS
50 mM Tris, pH 7.4

NP-40

NaCl 150 mM
NP-40 1%
Tris, pH 8.0 50 mM

HEPES

Hepes pH 7.4 50mM
NaCl 150mM
Glycerol 10%
Triton X-100 1%
KCl 5mM
EDTA 1mM
Protease inhibitors (leupeptin 10ug/ul, aprotinin 10ug/ul, PMSF 1mM)
Phosphatase inhibitors (NaF 50mM, Na3VO4 0.5mM, Na4P2O7 5mM)

Everything about western blots

Everything you always wanted to learn about western blots but were too afraid to ask is in this web site: http://www.westernblotting.org/
Western blot protocols, troubleshoting, definitions, buffers and theory all in a web site. What is biotin? how the stripping buffer works? how do I prepare the lysis buffer? In a very basic way, you’ll find your answers in this site.