Cell trypsinisation
1. Remove medium from culture dish. 2. Gently wash cells in a PBS without Ca or Mg. 3. Remove the wash solution. 4. Add trypsin-EDTA solution to cover the bottom of the culture dish (2ml for 10cm dish, 0.5ml for 60mm dish). 5. Put dish in the 37°C incubator for up to 5 minutes. 6. [...]