Everything about western blots

Everything you always wanted to learn about western blots but were too afraid to ask is in this web site: http://www.westernblotting.org/ Western blot protocols, troubleshoting, definitions, buffers and theory all in a web site. What is biotin? how the stripping buffer works? how do I prepare the lysis buffer? In a very basic way, you’ll find your answers in this [...]

Posted Under: Western blot
This post was written by admin on April 7, 2009 Comments (2)

Western blot protocol

A complete western blot protocol from Howel lab in University of California San Diego. Here is the link: http://cancer.ucsd.edu/howelllab/Western.html From how to obtain a cell lysate to how to prepare the gels, running the gels, membrane transfer, detection and stripping the membrane. Also all the reagents and buffers you need for your western blot. There is [...]

Posted Under: Buffers & recipes,Western blot
This post was written by admin on April 5, 2009 Comments (0)