Western blot troubleshooting

This post was written by admin on April 6, 2009
Posted Under: Western blot

Here some problems we all have had when doing western blots:

- high background signal
- strange/non specific bands on the blot
- low sensitivity
- is a right band detected?
- uneven results with lots of spots

In the protocols section of the company Agrisera there is a good troubleshooting section that will answer all your questions. Visit it here: http://www.agrisera.se/protocols/westerntrouble.shtml

Also, you can keep the link to take a look at the rest of the page later on, as it is pretty useful and everything it is good explained.

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