Western blot troubleshooting

This post was written by admin on April 6, 2009
Posted Under: Western blot

Here some problems we all have had when doing western blots:

- high background signal
- strange/non specific bands on the blot
- low sensitivity
- is a right band detected?
- uneven results with lots of spots

In the protocols section of the company Agrisera there is a good troubleshooting section that will answer all your questions. Visit it here: http://www.agrisera.se/protocols/westerntrouble.shtml

Also, you can keep the link to take a look at the rest of the page later on, as it is pretty useful and everything it is good explained.

Reader Comments

western blot having more dark background in positive sample sera, Could able to see bands in negative sera?

#1 
Written By Dr leena on July 18th, 2011 @ 3:21 am

Check out LI-COR’s blog on Western blot troubleshooting. We have lots of ideas to help you make your chemiluminescent or infrared fluorescent western blot great!

#2 
Written By Margaret Dentlinger on September 1st, 2011 @ 7:13 am

LI-COR blog address – licorbio.blogspot.com

#3 
Written By Margaret Dentlinger on September 1st, 2011 @ 7:14 am

Hi,
I’m doing WB targeting 8kDa molecule. I don’t know why I can’t get any bands on the x-ray film.
Any suggestions for handling 8kDa molueucle

#4 
Written By Basit on November 24th, 2011 @ 1:34 am

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